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Image Search Results


(A) Expression of KDR, encoding VEGFR2, and (B) FLT4, encoding VEGFR3, across publicly available pediatric solid tumor datasets included in the R2 MegaSampler platform. Expression values are presented as log2-transformed signal intensity. The distributions illustrate inter-dataset and inter-tumor heterogeneity in the expression of receptors associated predominantly with VEGF-A and VEGF-C signaling, respectively. M, metastasis; T, primary tumor; ES, Ewing sarcoma; NB, neuroblastoma; OS, osteosarcoma.

Journal: bioRxiv

Article Title: Computationally guided design of a metastasis-on-a-chip platform for quantitative evaluation of chemotactic cues in developmental cancers

doi: 10.64898/2026.07.25.740695

Figure Lengend Snippet: (A) Expression of KDR, encoding VEGFR2, and (B) FLT4, encoding VEGFR3, across publicly available pediatric solid tumor datasets included in the R2 MegaSampler platform. Expression values are presented as log2-transformed signal intensity. The distributions illustrate inter-dataset and inter-tumor heterogeneity in the expression of receptors associated predominantly with VEGF-A and VEGF-C signaling, respectively. M, metastasis; T, primary tumor; ES, Ewing sarcoma; NB, neuroblastoma; OS, osteosarcoma.

Article Snippet: VEGF-A165 and VEGF-C concentrations were quantified using commercially available human ELISA kits (RayBiotech, Peachtree Corners, GA, USA; Catalog Nos.

Techniques: Expressing, Transformation Assay

VEGF-C transport was simulated using initial concentrations of 0.1 or 5 µg/mL in Chamber 2. Predicted VEGF-C concentrations over time are shown in (A) Channel 2, adjacent to the source, (B) at the microchannel array separating Reservoirs 2 and 1, and (C) in Channel 1, on the Chamber 1 side of the device. An initial concentration of 5 µg/mL generated measurable exposure at the microchannel array within the experimental time window, whereas 0.1 µg/mL produced substantially lower concentrations and negligible predicted exposure at the microchannel array and in Channel 1. Different concentration units and y-axis ranges were used for the individual device regions.

Journal: bioRxiv

Article Title: Computationally guided design of a metastasis-on-a-chip platform for quantitative evaluation of chemotactic cues in developmental cancers

doi: 10.64898/2026.07.25.740695

Figure Lengend Snippet: VEGF-C transport was simulated using initial concentrations of 0.1 or 5 µg/mL in Chamber 2. Predicted VEGF-C concentrations over time are shown in (A) Channel 2, adjacent to the source, (B) at the microchannel array separating Reservoirs 2 and 1, and (C) in Channel 1, on the Chamber 1 side of the device. An initial concentration of 5 µg/mL generated measurable exposure at the microchannel array within the experimental time window, whereas 0.1 µg/mL produced substantially lower concentrations and negligible predicted exposure at the microchannel array and in Channel 1. Different concentration units and y-axis ranges were used for the individual device regions.

Article Snippet: VEGF-A165 and VEGF-C concentrations were quantified using commercially available human ELISA kits (RayBiotech, Peachtree Corners, GA, USA; Catalog Nos.

Techniques: Concentration Assay, Generated, Produced

Journal: bioRxiv

Article Title: Computationally guided design of a metastasis-on-a-chip platform for quantitative evaluation of chemotactic cues in developmental cancers

doi: 10.64898/2026.07.25.740695

Figure Lengend Snippet:

Article Snippet: VEGF-A165 and VEGF-C concentrations were quantified using commercially available human ELISA kits (RayBiotech, Peachtree Corners, GA, USA; Catalog Nos.

Techniques: Incubation, Cell Culture, Enzyme-linked Immunosorbent Assay

(A) Representative merged bright-field and fluorescence images of neuroblastoma, Ewing sarcoma, and osteosarcoma cells detected within the microchannel array under control, VEGF-A165, and VEGF-C conditions. Images were processed using a custom Fiji macro that automatically identified the microchannel region, detected fluorescent cells, and retained cells located within the microchannels according to predefined size, circularity, intensity, and channel-overlap criteria. Scale bars =50µm. (B) Quantification of the number of cells detected within the microchannels for neuroblastoma, Ewing sarcoma, and osteosarcoma. Individual data points represent independent microfluidic devices, and bars show mean ± SD. Statistical significance was assessed using ordinary one-way ANOVA followed by Dunnett’s multiple-comparisons test against the corresponding control. *p < 0.05; ns, not significant.

Journal: bioRxiv

Article Title: Computationally guided design of a metastasis-on-a-chip platform for quantitative evaluation of chemotactic cues in developmental cancers

doi: 10.64898/2026.07.25.740695

Figure Lengend Snippet: (A) Representative merged bright-field and fluorescence images of neuroblastoma, Ewing sarcoma, and osteosarcoma cells detected within the microchannel array under control, VEGF-A165, and VEGF-C conditions. Images were processed using a custom Fiji macro that automatically identified the microchannel region, detected fluorescent cells, and retained cells located within the microchannels according to predefined size, circularity, intensity, and channel-overlap criteria. Scale bars =50µm. (B) Quantification of the number of cells detected within the microchannels for neuroblastoma, Ewing sarcoma, and osteosarcoma. Individual data points represent independent microfluidic devices, and bars show mean ± SD. Statistical significance was assessed using ordinary one-way ANOVA followed by Dunnett’s multiple-comparisons test against the corresponding control. *p < 0.05; ns, not significant.

Article Snippet: VEGF-A165 and VEGF-C concentrations were quantified using commercially available human ELISA kits (RayBiotech, Peachtree Corners, GA, USA; Catalog Nos.

Techniques: Fluorescence, Control